Objective To translate and revise Hand Hygiene Behaviour Questionnaire（HHBQ） based on COM-B （capability,opportunity,motivation-behaviour） model,and to test its reliability and validity. Methods According to the intercultural debugging guidelines,the Chinese version of HHBQ was prepared by pre-translation,pre-translation synthesis,back-translation,back-translation synthesis,original author review,expert consultation and pre-test. The reliability and validity of Chinese version of HHBQ were evaluated by a survey of 537 medical staff from 3 tertiary A hospitals in Hangzhou from July to October 2021. Results The Chinese version of HHBQ contained 3 dimensions,namely,hand hygiene ability（5 items）,hand hygiene opportunity（7 items）,and hand hygiene motivation （5 items）. The content validity of the total scale was 0.995,and the content validity of each item in the questionnaire was 0.923~1.000. 3 common factors were extracted from exploratory factor analysis,and the cumulative variance contribution rate was 68.741%. Confirmatory factor analysis showed that the model fit well. The Cronbach’s α coefficients of the total amount table are 0.918,and the Cronbach’s α coefficients of each dimension are 0.871~0.906. The retest reliability of the questionnaire was 0.739. Conclusion The Chinese version of HHBQ has good reliability and validity,and it can be used to comprehensively evaluate the influencing factors of medical staff’s hand hygiene.